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ahm[at]bwh.harvard.edu
Molecular Imaging in the Retina
Retina with fluorescent microspheres

Our imaging approach is inspired by the innate immune response ubiquitous in many pathologies. We developed fluorescent nano-probes with a variety of surface moieties that mimic leukocyte interaction with the inner lumen of the vascular endothelium. When injected into the blood stream of live animals, these nano-probes circulate throughout the animal’s vasculature. More>>

Macrophages Differentiation

Resident and recruited macrophages polarize into M1 and M2 subtypes, two extremes in a continuum of polarization states. We introduced the role of ROCK signaling in macrophage polarization. The rho-associated, coiled–coil–containing protein kinases (ROCKs) are cytosolic signaling molecules that contribute to cytoskeletal functions and contractility. More>>

neovasculariation in cornea
Novel Micro-fluidic System
Novel Micro-fluidic System

To study the interaction of native mouse blood cells with individual immobilized proteins, we developed the autoperfused mouse flow chamber.

In this experimental system we micro-surgically attach a custom-designed micro-flow chamber assembly to the carotid artery and jugular vein of a live mouse under anesthesia. More>>

The Inner and Outer Blood-Retina-Barrier

The method of choice for measurements of trans-epithelial electrical resistance (TEER) ex-vivo is the Ussing-chamber, named after the Danish physiologist Hans Ussing (1911-2000). His work together with Zerhahn in the 1950s laid the foundation for our understanding of polarized cells.

An Ussing chamber is comprised of two half chambers, which are filled with a buffer, for instance Ringer’s solution. We managed to micro-surgically isolate the retinal pigment epithelium (RPE) from the rat eye, which is ~2 mm in diameter. More>>

The Inner and Outer Blood-Retina-Barrier
Type 2 Diabetes
Diabetic Aorta

To overcome the limitations of existing models of diabetes, our team together with our collaborator, Dr. KC Hayes at Brandeis University, introduced the nile grass rat (NGR) as a unique animal model that recapitulates all relevant organ manifestations of diabetes. More>>

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